Reagents and enzymes

The restriction endonucleases used in this study were purchased from Thermo Fisher Scientific (Waltham, USA). DNA polymerases, including 2×Phanta Flash Master Mix (Vazyme Biotechnology Co., Ltd., Nanjing, China) for high-fidelity DNA amplification and 2×Es Taq MasterMix (Dye) (Jiangsu Cowin Biotech Co., Ltd., Taizhou, China) for colony PCR, were used. A reagent kit for plasmid extraction was obtained from Tiangen (Tiangen Biotech Co., Ltd., Beijing, China), and a reagent for DNA purification was obtained from TransGen (Beijing, China) following the manufacturer’s instructions. A ClonExpress One Step Cloning Kit (Vazyme Biotechnology Co., Ltd.) was used for the assembly of plasmids.