Mouse genotyping

For genotyping of the Lmna alleles, genomic DNA of isolated cardiomyocytes was extracted with Phenol/Chloroform/Isoamyl alcohol (Invitrogen,15593–031), treated with RNaseA and Proteinase K, and purified. Floxed, recombined, and wild type Lmna alleles were detected by PCR using the primers KI279 and KI280 (Table S3). Based on the agarose gel (1.5%) analysis of PCR-amplified products, recombination efficiency (%) was calculated by 153-bp band (Recombined) over 598-bp band (Floxed) intensities. Genotyping of Myh6-MerCreMer, Cgas, Sting, Uprt, and Rosa26::CAG-LSL-tdTomato alleles was performed at Transnetyx, Inc.