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Further gene expression analysis

IK Ivan V. Kuzmin
RA Ruben Soto Acosta
LP Layne Pruitt
PW Perry T. Wasdin
KK Kritika Kedarinath
KH Keziah R. Hernandez
KG Kristyn A. Gonzales
KH Kharighan Hill
NW Nicole G. Weidner
CM Chad Mire
TE Taylor B. Engdahl
WM Woohyun J. Moon
VP Vsevolod Popov
JJ James E. Crowe, Jr
IG Ivelin S. Georgiev
MG Mariano A. Garcia-Blanco
RA Robert K. Abbott
AB Alexander Bukreyev
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Only IgG-expressing B cells were retained for further analysis, as these B cells are expected to be elicited by the vaccine treatments, and differences in gene expression between B cell subtypes could convolute the downstream analyses. Differential gene expression (DGE) analysis was performed to identify differentially expressed genes between the cells from mice in the U-mRNA and m1Ψ-mRNA groups based on Benjamini–Hochberg adjusted p values. DGE was also performed between Cluster 9 cells and the rest of the matrix to identify upregulated genes in this cluster (Fig. S4).

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