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The Y2H assays were conducted according to previously described methods67. In brief, pGADT7-LjLHW and pGBKT7-LjNIN-PB1 were co-transformed into yeast strain AH109 (Clontech), or transformed separately with the complementary empty vector. The yeast transformants were screened on SD (Synthetic Dropout) media lacking leucine and tryptophan (SD/-Leu-Trp). Protein interaction and self-activation were assessed by screening yeast transformants on SD media lacking leucine, tryptophan, histidine and adenosine (SD/-Leu-Trp-His-Ade).
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