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Frozen sections of decidual tissue were fixed in 4% PFA for 1 h and washed three times for 5 min each with 0.05% PBS-Triton X-100. BSA blocking solution (5%) was then applied to the slides for 1 h at RT to block nonspecific binding within the tissue. We added primary antibodies to the sections and incubated them overnight at 4 °C, and secondary antibodies and DAPI were added and incubated for 1 h at RT (the antibodies used in this study are listed in Supplementary Table 2).
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