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Neutrophils were washed with PBS, suspended in 200 μL of binding buffer, and stained with 10 μL of Annexin V-FITC and 10 μL of propidium iodide (PI). After staining for 15 min in the dark, 500 μL of binding buffer was added. The samples were then assayed via flow cytometry (BD Accuri C6, USA). The excitation wavelength of the argon laser was set at 488 nm.
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