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Intra-cellular staining
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Cells were cell-surfaced stained and fixed overnight with 1% PFA prior to permeabilization with 0.3% Saponin (Sigma-Aldrich) for 15 mins at 4 °C. Cells were wash with 0.1% Saponin, incubated with specific antibodies or matched isotype controls for 30 mins at 4 °C. Cells were wash with PBS and flow cytometry analysis was performed immediately using the CytoFLEX S.
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