For clonogenic experiments with SUM149PT and OVSAHO cells, 500 cells were plated in technical replicates in 6-well plates and analyzed after 10–14 days. For clonogenic experiments with potassium bromate, cells were plated on Day 0, potassium bromate was added on Day 1, and media was changed on Day 8. For clonogenic experiments with UV, cells were plated on Day 0, treated with UV on Day 2, and media was changed on Day 9. In each of these experiments, after 10–14 days, cells were washed with PBS and stained with 0.4% crystal violet in 20% methanol for 30 min at room temperature. Plates were then washed with deionized water and air-dried. Colonies were manually counted.
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