MTT assay

Cell lines KASUMI-1 and MV-4-11 were seeded in a 96-well plate at a concentration of 1 × 10⁵ cells/mL, 16 h after seeding they were treated based on bibliographic data with Idarubicin and Cytarabine in order to conduct the gene expression profiling. MOLM-13 and KASUMI-1 cells were seeded in a 96-well plate at a concentration of 1 × 10⁵ cells/mL. Afterward, they were treated with Idarubicin or Cytarabine at a concentration range of 1nM- 10µΜ (with a 10fold gradual increase) and were further incubated for 24, 48, and 72 h. TF-1 cells were seeded in a 96-well plate at a concentration of 1 × 10⁵ cells/mL. Afterward, they were treated with Idarubicin (0.01, 0.05, 0.1, 0.2, and 0.5µΜ) or Cytarabine (0.1, 0.5, 01, 2, 5µΜ) and were further incubated for 24, 48 and 72 h. The colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay was used for cell viability determination at each time-point and for each agent concentration. MTT (Sigma, USA) was dissolved in PBS at 5 mg/mL, the MTT solution was added to the 96-well plate at volume 20 µL, and the resulting solution was incubated in 5% CO2 for another 4 h at 37 °C. Formazan crystals were dissolved in 200 µL of SDS-HCL. The plates were then analyzed in a plate reader at 570 nm. All experiments were performed in triplicate.