Detection of EGFR hotspot mutations using droplet digital PCR (ddPCR)

AR Amber Rathor
PM Prabhat Singh Malik
PT Pranay Tanwar
SK Sachin Khurana
HB Hemavathi Baskarane
DP Deepam Pushpam
AN Aruna Nambirajan
DJ Deepali Jain
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All droplet digital PCR (ddPCR) consumables including droplet PCR supermix, droplet generation oil for probes, droplet generator cartridges and gaskets, droplet reader oil and ddPCR 96-well plates were procured from Bio-Rad Laboratories Inc. (Hercules, CA, USA). ddPCR was performed with three commercially available PrimePCR™ ddPCR™ Mutation Detection Assay Kit for E746_A750del, L858R and T790M (Bio-Rad; Hercules, CA). The PCR reaction was performed according to the manufacturers’ instructions and the droplets were read by Bio-Rad QX200 ddPCR droplet reader system and finally analysed using Quantasoft version 1.7.

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