All droplet digital PCR (ddPCR) consumables including droplet PCR supermix, droplet generation oil for probes, droplet generator cartridges and gaskets, droplet reader oil and ddPCR 96-well plates were procured from Bio-Rad Laboratories Inc. (Hercules, CA, USA). ddPCR was performed with three commercially available PrimePCR™ ddPCR™ Mutation Detection Assay Kit for E746_A750del, L858R and T790M (Bio-Rad; Hercules, CA). The PCR reaction was performed according to the manufacturers’ instructions and the droplets were read by Bio-Rad QX200 ddPCR droplet reader system and finally analysed using Quantasoft version 1.7.
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