RAW264 cells were treated with 1 ug/mL of M. oleifera hexane extract and celecoxib drug for 24 h. After incubation, the supernatant was collected and used for NO assay. In the case of the NO assay, the supernatants were mixed with Griess reagent containing equal volumes of 1% sulfanilamide (Sigma Aldrich Co., St. Louis, MO) in 2.5% phosphoric acid and 0.1% naphthylethylenediamine dihydrochloride (Sigma Aldrich Co., St. Louis, MO) solution and then incubated at room temperature for 10 min. The concentration of nitric oxide was measured by OD at 595 nm. Sodium nitrite (NaNO2) (Sigma Aldrich Co., St. Louis, MO) was used as a standard regent.
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