4.2. Purification and Characterization of the Peptides

The synthesized peptides were purified by high-performance liquid chromatography (HPLC) on a Shimadzu chromatograph in semipreparative mode on a C18 reversed-phase column (Phenomenex 2.1 × 25 cm), according to Table S1. The degree of purity of the fractions was determined on a C18 reversed-phase Shimadzu chromatograph on an analytical column (0.46 × 25 cm) of (Kromassil) in a gradient of 5–95% of solution B in 30 min, with a flow of 1 mL·min⁻¹. The profiles of the pure peptides are shown in Figure S1. The solutions used were 0.045% TFA in ultrapure water (solution A) and 0.036% TFA in acetonitrile (solution B).