2.1.2. In Vitro Metabolism Data

David Bibi; Raphael Bilgraer; Lilach Steiner; Hussein Hallak

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

2.1.2. In Vitro Metabolism Data

DB David Bibi

RB Raphael Bilgraer

LS Lilach Steiner

HH Hussein Hallak

This method is extracted from research article: Pharmaceutics, Jul 2024

Physiologically-Based Pharmacokinetic Modeling and In Vitro–In Vivo Correlation of TV-46000 (Risperidone LAI): Prediction from Dog to Human

DOI: 10.3390/pharmaceutics16070896

Ask a question

Favorite

To characterize the metabolism of risperidone, an in vitro phenotyping assay was conducted using human recombinant cytochromes P450 (rCYP) 2D6 and 3A4. The formation of risperidone active metabolite, 9-OH-risperidone, was measured over time, and the Michaelis–Menten constants K_m and V_m were calculated for each rCYP.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol