Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.6. ELISA

JW Jinzhi Wang
YY Yuanyuan Yao
TY Ting Yao
QS Qingmiao Shi
YZ Yifan Zeng
LL Lanjuan Li
request Request a Protocol
ask Ask a question
Favorite

To acquire serum, the blood of mice was collected using the capillary tube from the ophthalmic venous plexus and placed at room temperature for one hour before centrifugation at 3000 rpm, 4 °C for 15 min. For the colitis model in vitro, the supernatant of cells in different groups was collected for detection. The inflammatory factors (TNFa and IL6) were examined following the instructions of the ELISA Kits (Liankebio, Hangzhou, China).

Copyright and License information:  ©2024 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A