2.1. Reagents and Instruments

HT was purchased from MedChemExpress (Shanghai, China). Dextran sulfate sodium salt (DSS, M.W 40,000) and carboxymethyl cellulose sodium (CMC-Na) were bought from Macklin (Shanghai, China). Anti-GAPDH Mouse Monoclonal antibody (mAb) was bought from Servicebio (Wuhan, China). ACSL4 rabbit mAb was from ABclonal (Wuhan, China). GPX4 mouse mAb and PTGS2 mouse mAb were both from Proteintech (Wuhan, China). HRP-labeled Goat Anti-Rabbit IgG (H + L) and HRP-labeled Goat Anti-Mouse IgG (H + L) were from Beyotime (Shanghai, China).