2.11. Sensitivity Test for the Dual Fluorescence RT-RAA

To verify the sensitivity, the plasmid constructed by the Y10 strain S gene and CK/CH/GX/NN16-2 strain S gene was mixed with a ratio of 1:1. This mixture was then diluted using a 10-fold ratio into 6 groups of plasmids with different concentrations: 10⁰, 10¹, 10², 10³, 10⁴, and 10⁵ copies/μL. A dual fluorescence RT-RAA reaction was performed with 6 groups of different concentrations of plasmids as templates, and ddH₂O was used as blank control. The test was repeated 3 times.