2.4.3. In Vivo Biochemical Parameters Analysis

The concentration of lipids (lipid status) was determined in the serum of animals. The concentrations of total cholesterol, total triglycerides, and high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) were measured using clinical biochemistry methods.

The enzymatic activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), as well as the concentrations of urea and creatinine, were assessed in order to monitor hepatic and renal function using serum of the examined animals.

All analyses were performed using standard spectrophotometric methods on an automatic chemical analysis system, Olympus AU 400 (Hamburg, Germany).