2.4. Male Fertility Test

To evaluate the reproductive ability of male Fd59a mutant flies, a male fertility test was conducted. Ten 1-day-old virgin w¹¹¹⁸ females were housed with fifteen 3-day-old w¹¹¹⁸ or Fd59a^2/2 males in vials containing egg collection plates to collect eggs. The egg collection plates were replaced every 24 h, and the number of embryos and larvae in the plates was counted as described previously [24].