4.9. Cell Differentiation Assays

To evaluate cell differentiation, morphology and CD11b and CD14 expression were detected. The expression of the cell surface differentiation-related antigens CD14 and CD11b was determined by flow cytometry. Fluorochrome-labeled anti-human CD14/FITC and anti-human CD11b/PE antibodies were purchased from BioLegend (San Diego, CA, USA). After the desired treatment, U937 cells were suspended in FACS buffer (1X PBS containing 1% FBS) and stained for 1 h at room temperature in the dark. The data were collected on LSRFortessa (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Morphology was determined using May-Grunwald-Giemsa staining (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol. The cells were centrifuged into slides by cytospin (Sigma Biomolecules, St. Louis, MO, USA) and viewed at ×200 magnification.