4.8.4. Western Blot Studies for p-p38 MAPK

Levels of phosphorylated p38MAPK in mouse kidney homogenates were semi-quantified with Western blotting using an anti-phospho-p38MAPK antibody (Cell Signaling Technologies, Danvers, MA, USA) as described in [32]. Membranes were imaged using a ChemiDoc Touch system (BioRad, Sydney, Australia) and densitometric analysis conducted with ImageLab^™ software (v 6.0.1, 2017, BioRad, Sydney, Australia). All data were normalised against total protein (bands detected in situ) using the corresponding membranes. Changes in p38MAPK activation were expressed relative to the control.