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An MTT assay was performed to determine the number of viable cells in culture. Different concentrations (0, 1, 10, 25, 50, 100, and 200 µM) of HSYA were added to keratinocytes. The cells were incubated for 24 h. The absorbance was measured at 570 nm in a plate reader to determine the formazan concentration, which is proportional to the number of living cells in culture.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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