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2.5. Enzymatic Activity Assay

SW Suwakon Wongjaikam
CN Chutikorn Nopparat
PB Parichart Boontem
JP Jiraporn Panmanee
NT Nopporn Thasana
MS Mayuri Shukla
PG Piyarat Govitrapong
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Activity assays for BACE1 (β-secretase) and ADAM10 (α-secretase) were carried out according to the manufacturer’s instructions (AnaSpec, Fremont, CA, USA). The final concentrations of Hup A were 0.1, 1, and 10 μM. Inhibitors of BACE1 (LY2886721) or ADAM10 (GM 6001) obtained from the assay kits were employed for the experimental positive group (positive control). The fluorescence intensity was measured in a Synergy HTX multimode reader (BioTek, Winooski, VT, USA) at excitation/emission wavelengths of 490/520 nm.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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