Study of the anti-potency of VFLE against C. fructicola

VFLE at various concentrations was treated in the culture medium where the C. fructicola fungus was then inoculated. The impact of VFLE on the parasitic mycelium of C. fructicola was evaluated by quantifying colony growth. The study used a PDB medium prepared using 3.5 g of potato dextrose agar + 100 mL of distilled water, sterilized at 121 °C for 20 min. Once cooled, VFLE at final concentrations of 0, 100, 200, 400, 600, 800, and 1000 µg/mL, respectively, was added. Fungal cubes (6 mm) of C. fructicola were placed and the medium was then incubated at 28 °C. Each test involved three repeats.