Silencing of mouse talin in T cells was achieved by shRNA. Recombinant lentiviruses expressing scramble shRNA (5’-CCTAAGGTTAAGTCGCCCTCG-3’) or shRNAs that annealed to talin (shTalin-1#: 5’-GCAGAAGGGAGAGCGTAAGAT-3’; shTalin-2#: 5’-GAAGCACAGAGCCGATTGAAT-3’) were used. The silencing of talin was confirmed by immunoblotting 48 h post-transfection.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.