Silencing of talin in T cells

YL Yue Li
SW ShiHui Wang
YZ YouHua Zhang
ZL ZhaoYuan Liu
YZ YunZhe Zheng
KZ Kun Zhang
SC ShiYang Chen
XL XiaoYing Lv
MH MengWen Huang
XP XingChao Pan
YZ YaJuan Zheng
MY MengYa Yuan
GG GaoXiang Ge
YZ Yi Arial Zeng
CL ChangDong Lin
JC JianFeng Chen
request Request a Protocol
ask Ask a question
Favorite

Silencing of mouse talin in T cells was achieved by shRNA. Recombinant lentiviruses expressing scramble shRNA (5’-CCTAAGGTTAAGTCGCCCTCG-3’) or shRNAs that annealed to talin (shTalin-1#: 5’-GCAGAAGGGAGAGCGTAAGAT-3’; shTalin-2#: 5’-GAAGCACAGAGCCGATTGAAT-3’) were used. The silencing of talin was confirmed by immunoblotting 48 h post-transfection.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A