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Briefly, preparations of fixed cells were rinsed three times with PBS, permeabilised with 70% ethanol (30 s), and incubated with a solution of Hoechst 33258 (2 μg/mL) for 30 min at room temperature (RT). Then, the cells were observed by fluorescence microscope (Leica, Germany).
Copyright and License information: ©2016 Miao Su et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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