Mouse model of hepatic ischemia‒reperfusion and drug treatment

The mice were anesthetized with isoflurane, the skin was disinfected, and a median abdominal incision was made in the abdominal cavity to separate the portal vein branches and hepatic artery of the hepatic pedicle of the left and middle lobes of the liver. The portal vein branches and hepatic arteries of the hepatic pedicle of the left and middle lobes of the liver were clamped with a noninvasive vascular clamp. After continuous ischemia for 1 h, the vascular clamp was released to restore blood flow.⁴³ The mice were anesthetized at 0, 6, 12, and 24 h after reperfusion. Blood and liver samples were taken and preserved until further use. The Pim-1 inhibitor (TCS Pim-1 1) HY-18086 (MedChemExpress) was dissolved in 0.5% DMSO.⁴⁴ The mice were randomly divided into three groups: a sham operation group, a control group and an experimental group. The sham group was intraperitoneally injected with 0.5% DMSO solution. The control group was intraperitoneally injected with 0.5% DMSO solution for 5 days, while the experimental group was intraperitoneally injected with 5 mg/kg TCS PIM-1 for 5 days, and then the control and experimental groups were used to establish the mouse hepatic IR model.