Human liver samples (formalin-fixed, paraffin embedded, n = 21; ethical approval: 518/19 S) were double-stained by RNAscope (CXCR6) and CD3 (MRQ39, 1:1,500). Briefly, after deparaffinization and standard pretreatment, slides were incubated with RNA probes for CXCR6 (468468, ACD, Bio-Techne), detected with a RNAscope 2.5 Leica Assay-brown (Leica Biosystems) followed by incubation with a primary antibody against CD3 (103R-95, CellMarque) and detection with a Bond Polymer Refine Red Detection Kit (Leica Biosystems) on a Bond Rxm system (Leica Biosystems). All slides were counterstained with haematoxylin, cover slipped and digitalized using an AT2 scanner (Leica Biosystems). The study was conducted according to federal guidelines, local ethics committee regulations of the Technical University of Munich, Germany (no. 518/19 S-SR)
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