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The mRNA was extracted from LNP as previously reported.33 Briefly, mRNA-LNP was mixed with 60 mM ammonium acetate (Sigma) diluted in isopropanol at a 1:9 ratio. The mixture was centrifuged at 16,000× g at 4°C for 15 min, and the pellet was washed with isopropanol. The solution was then centrifuged again for 5 min. The extracted mRNA pellet was resuspended in Ambion Nuclease-Free Water (Invitrogen) and quantified by NanoDrop spectrophotometer (Thermo Scientific).
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