Extraction and Quantification of α-Bisabolene

The dodecane phase of the cell culture was collected and subsequently subjected to gas chromatography (GC) to analyze the α-bisabolene concentration using caryophyllene as an internal standard. The GC (Thermo Scientific TRACE 1300) was equipped with a flame ionization detector (FID) and an HP-5 column (30 m × 0.320 mm × 0.25 μm) with a flow rate of 1.0 mL/min. The injection volume was 1.0 μL with a split ratio of 15, and the temperature of the injector and GC interface was 250 °C. The initial temperature was 60 °C, followed by a ramp to 200 °C at a rate of 40 °C/min and holding at 200 °C for 5 min.