2.6. Histopathology of liver tissue

The following steps were used to perform histopathological analysis. Briefly, the rats were dissected to remove the liver after the completion of experiment, the liver was removed from each animal, washed with normal saline, and preserved in 10% formalin for fixation. The samples of liver were cut into small section of about 5 μm for post fixation. The tissue samples were kept at different concentrations of ethanol, xylene, and paraffin for dehydration and cleaning. The specimen was embedded in paraffin wax to solidify, and the blocks were preserved in refrigeration to avoid contamination. The samples were cleaned with xylene to perform sectioning. Mayer's egg albumin was used to fix the sample on the glass for mounting. The tissue was stained with the help of hematoxylin and eosin for conducting staining (Slaoui et al., ²⁰¹⁷). The liver samples were sent to Pathology Department, Faisalabad Medical University, Faisalabad, Pakistan, for histopathological studies (Lab slides no: 3139). The slides were observed under light microscope in the Pharmacology and Physiology departments, University of Agriculture, Faisalabad.