2.9. Histopathological analysis

For histopathological analysis, cross‐sections of liver were prepared and examination was done through methods described by Donald et al. (²⁰¹⁵). For that purpose, animals were sacrificed, and then liver and stomach sections were placed in 10% formaldehyde and washed with xylene. These sections were enclosed in wax made up of paraffin and with the help of a rotary microtome and were suctioned up to 5 μm thickness. After this, hematoxylin and eosin stainings were performed. Then, the light microscope was used to analyze the sections of the liver and stomach, and microscopic camera was used to take photograph (Donald et al., ²⁰¹⁵).