Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Live/dead cell staining

YZ Yanfeng Zhao
XD Xinyi Dong
YL Yang Li
JC Juan Cui
QS Qing Shi
HH Hen-Wei Huang
QH Qiang Huang
HW Huaping Wang
ask Ask a question
Favorite

Live/dead staining assays of the cell encapsulation within the hydrogel microstructures were performed by labeling with calcein acetoxymethyl ester (2 μg/ml) and propidium iodide (3 μg/ml) fluorescent dyes (Molecular Probes, Eugene, OR, USA). Cellular viability within the hydrogel microstructures was assessed daily using ImageJ software to analyze the areas of red fluorescence (dead cells) and green fluorescence (live cells) in each image. Cellular viability was quantified by calculating the ratio of the area of green fluorescence relative to the total area of fluorescence.

Copyright and License information:  ©2024 Yanfeng Zhao et al.
Exclusive licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A