2.4.3. Determination of inflammatory marker levels

TNF‐α (Cat. No. E‐EL‐M3063), IL‐1β (Cat. No. E‐EL‐M0037), IL‐6 (Cat. No. E‐EL‐M0044) and IL‐10 (Cat. No. E‐EL‐M0046) levels in brain tissues were measured using commercial ELISA kits (Elabscience, USA) according to the manufacturer procedures. Optical density (OD) was quantified spectrophotometrically at 450± 2 nm. The OD value is proportional to the mouse TNF‐α, IL‐1β, IL‐6 and IL‐10 concentrations. Inflammatory cytokines levels in the samples were calculated by comparing the optical density of the samples with the standard curve.