- Home
- Protocols
-
Request a Protocol
Ask a
question
MAIT cells, identified as Vα7.2+ CD161+ cells, and CD4+ CD25− responder T cells were sorted from the peripheral blood of 3 healthy donors. Responder T cells were labeled with 0.5 μM CFSE. 2.5×104 CFSE-labeled responder T cells were stimulated with Dynabeads CD3/CD28 T Cell Expander (Dynal Biotech) and cultured with MAIT cells or responder T cells at a ratio of 1:1, 1:2, 1:4, and 1:8 MAIT:responder in round-bottom 96-well plates for 4 days. After 4 days, CFSE dilution was evaluated by flow cytometry.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
