3.12 Endogenous biotin blocking

Eliseo A. Eugenin; Joan W. Berman

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3.12 Endogenous biotin blocking

EE Eliseo A. Eugenin

JB Joan W. Berman

This method is extracted from research article: Methods Mol Biol, Jan 2016

Improved methods to detect low levels of HIV using antibody-based technologies

DOI: 10.1007/978-1-4939-3046-3_18

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If the tissue has endogenous biotin activity, biotin blocking is suggested using a Biotin blocking kit. First, incubate the tissue sections or pelleted leukocytes with avidin solution for 10–30 minutes and then wash in TBST 3 times for 5 minutes. Next, incubate with biotin solution for 10–30 minutes and then wash in TBST 3 times for 5 minutes.

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