Feeding regimes

3 week HFD: male 6–8 week old WT C57BL/6 mice were fed a HFD or regular control diet for 3 weeks. 9 week HFD: male and female mice 3 week old Winnie mice⁷ on a C57BL/6 background and aged matched WT controls were placed on a HFD or NCD for 9 weeks, 22 week HFD (Additional experimental details and metabolic data was reported previously⁸): Male 6–8 week old WT C57BL/6 mice were fed a HFD or NCD for 22 weeks, and after 18 weeks, high dose (100 ng/g) or low dose (20 ng/g) recombinant IL-22 (Cell Signalling), or phosphate buffered saline (PBS) as a vehicle control, were administered by intra-peritoneal injection twice weekly for 4 weeks. HFD feeding continued during this period - mice were sacrificed after 22 weeks (data shown in Figs 1,​,22,​,66 and ​and77).