CUT&Tag

The CUT & Tag experiments were performed exactly following the online protocol⁵³: https://www.protocols.io/view/bench-top-cut-amp-tag-z6hf9b6?version_warning=no. For each targeted protein, 0.1 million cells were used. The following primary antibodies were used with 1:50 dilution: CTCF (Active motif 61311), H3K27ac (Active motif 39133), H3K27me3 (Cell signaling C36B11), dCas9 (Sigma-Aldrich #SAB4200701), Rabbit IgG (Cell signaling 2729). We use Guinea Pig anti-Rabbit IgG (H+L) secondary antibody (NBP1-72763) with 1:50 dilution. pA-Tn5 was bought from The earlier batch pA-Tn5 fusion protein was kindly gifted to us from Dr Steven Henikoff’s Lab. Final libraries were sequenced as 150 bp paired-end reads on platform Novaseq or Hiseq Xten with raw sequencing depths between 10 to 20 million read pairs.