NCI-H1703 Subcutaneous Xenograft Tumor Mouse Model

A total number of 40 homozygous NOD.CB17 Prkdc^scid/NCrHsd mice were used for the xenograft tumor model. On the day of implantation, NCI-H1703 cells were harvested and suspended at a concentration of 1 × 10⁸ cells per mL in an equal mix of Cultrex:RPMI without supplements. A volume of 100 μL was injected into the right hind flank of each animal. Tumor volumes were monitored until mean tumor volume reached 150 mm³. Then, mice were stratified into four treatment groups of each 10 mice and orally dosed twice per day with either EVT801 30 mg/kg and EVT801 vehicle [Soluplus (BASF)/water/hydroxypropylcellulose SL (Nisso America)], or 30 mk/kg pazopanib and pazopanib vehicle (0.5% Hydroxypropyl methylcellulose trimellitate (HPMCT) + 0.1% Tween-80). A period of 8 hours was observed between the first and second daily dose.