Bilateral subdiaphragmatic truncal vagotomy and pyloroplasty

Mice were anaesthetised with ketamine (100 mg kg⁻¹) and xylazine (10 mg kg⁻¹) i.p. A midline abdominal incision was made and the vagotomy was performed by dividing and cutting both vagal trunks immediately below the diaphragm. To prevent food retention and fatal gastric dilatation a pyloroplasty was performed by cutting the pylorus lengthwise and closing it in a perpendicular fashion using two single 8–0 non-absorbable sutures. The abdominal cavity was closed in a two layer fashion. In control animals (Sham operation), the subdiaphragmatic vagal nerves were isolated but not cut and a pyloroplasty was performed.