2.2. Preparation of Hydroalcoholic Extract of Peganum harmala

The seeds of Peganum harmala were collected from Zahedan in 2014. Plant was identified and authenticated by Botany Department of Sistan and Baluchestan University. Powdered seed of Peganum harmala (20 g) was placed in Soxhlet system with ethanol (70%) for 8 hours. Then extract dried in incubator (37°C) to give a final yield of approximately 10% hydroalcoholic extract of seeds. In time of experiments, the extract was dissolved in normal saline and animals were force-fed with above-mentioned doses.