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Luciferase Assay

AK Adam Kosti
JC Jennifer Chiou
GG Gabriela D A Guardia
XL Xiufen Lei
HB Henriette Balinda
TL Tesha Landry
XL Xiyuan Lu
MQ Mei Qiao
AG Andrea Gilbert
AB Andrew Brenner
PG Pedro A F Galante
ST Stefano Tiziani
LP Luiz O F Penalva
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293T cells were plated into 96-well plates and transfected with the ELF4 3′UTR construct16 using Lipofectamine 3000 (ThermoFisher) along with miRNA mimics or control oligos. Firefly luciferase luminescence was measured 48 h later using the Dual-Glo® Luciferase Assay System (Promega). Sea pansy luminescence was measured as a transfection control.

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