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Measurement of ciliary beat frequency
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Four ciliary beat recordings of 2–3 sec each were made at 60-sec intervals at a speed of 200 frames/sec using the high-speed digital imaging system HAS-U1 (DITECT, Tokyo, Japan) and were analyzed by HAS-XViewer Camera Memory ver. 1.2.12 (DITECT). The number of ciliary beats was counted manually by checking the video in a slow replay mode. CBF was measured at three different sites of each mucosal strip. The CBF value in each experiment was determined by averaging the 12 measurements (4 recordings×3 sites).
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