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High-Performance Liquid Chromatography (HPLC) Analysis
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Cogent Bidentate C18 column (4.6 mm × 250 mm, 5 μm particle size; microSolv Technology Corp., USA) was used and 10 μl of the reaction sample was injected. Elution was performed at 30°C using a 9:1 ratio of acetonitrile and water at a flow rate of 1 ml/min. Eluted material was analyzed with a refractive index detector (Agilent 1100 HPLC G1362A) at 35°C. Fig. S1 shows results of HPLC analysis for each alkyl butyrate synthesized in reactions.
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