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Malondialdehyde (MDA) assay

SQ Sidra Qurat-ul-Ain
AR Anwar Rukhsana
ST Sahar Isma Tariq
AK Ashiq Kanwal
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Malondialdehyde (MDA) test was carried out by using thiobarbituric (TBA) assay followed by Ohkawa method with a slight alteration in the experimental steps. Heart and brain tissue samples (1mg) were homogenized in 1.2% KCl separately. To homogenate, 20% acetic acid, 8% sodium lauryl sulfate, 20.8% TBA, and distilled water were added. The reaction mixture was incubated for 1 hour at 98°C. After incubation, butanol: pyridine (15:1) was added to the mixture and centrifuged for 30 min (4,000 rpm). The absorbance of the resultant supernatant was recorded at 532nm. The concentration of MDA (mM/g tissue) was directly calculated from the MDA standard curve.30

Copyright and License information:  ©2022 Qurat-ul-Ain S et al.
Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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