CRC cell lines were obtained from the American Type Culture Collection (ATCC) and grown in a medium supplemented with 10% foetal calf serum I (Thermo Fisher Scientific, Waltham, MA, USA). SW480 cells were grown in Leibovitz’s L-15 medium (ATCC, Manassas, VA, USA). HCT116 cells were grown in high-glucose DMEM medium (Thermo Fisher Scientific, Waltham, MA, USA). RKO and Caco2 cells were grown in ATCC-formulated Eagle’s Minimum Essential Medium (ATCC, Manassas, VA, USA).
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