Cell culture

AS Alexei J. Stuckel
SZ Shuai Zeng
ZL Zhen Lyu
WZ Wei Zhang
XZ Xu Zhang
UD Urszula Dougherty
RM Reba Mustafi
TK Tripti Khare
QZ Qiong Zhang
TJ Trupti Joshi
MB Marc Bissonnette
SK Sharad Khare
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CRC cell lines were obtained from the American Type Culture Collection (ATCC) and grown in a medium supplemented with 10% foetal calf serum I (Thermo Fisher Scientific, Waltham, MA, USA). SW480 cells were grown in Leibovitz’s L-15 medium (ATCC, Manassas, VA, USA). HCT116 cells were grown in high-glucose DMEM medium (Thermo Fisher Scientific, Waltham, MA, USA). RKO and Caco2 cells were grown in ATCC-formulated Eagle’s Minimum Essential Medium (ATCC, Manassas, VA, USA).

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