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Cell proliferation assay

CC Chen Chen
QZ Qing Zhang
BK Beihua Kong
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Twenty-four hours after siRNA or NC transfection, 1000 transfected cells in 100 μL of medium were transferred into each well of 96-well plates and incubated until the cells adhered. Cell proliferation was measured every 24 h for a total of 5–7 days. Then, 20 μL of 5 mg/mL MTT (Sigma-Aldrich; Missouri, USA) was added into each well at a fixed time and the cells were incubated for another 4 h. Next, 100 µL DMSO (Sangon Biotech, Shanghai, China) was added to each well after removing the supernatant carefully. Subsequently, the absorbance value at 490 nm was detected by a Varioskan Flash microplate reader (Thermo Fisher Scientific, USA). All the experiments were performed in triplicate.

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