Ethanol Self Administration

After completing retesting, rats remained undisturbed in their home cages for 20 days before the start of ethanol self-administration (SA; Fig. 1a). During the 15 sessions of ethanol SA, rats were taken to a separate testing room and placed in individual cages containing a bottle of water and three sipper tubes that each contained 5 mL of a sweetened ethanol solution (10% v/v ethanol and 0.2% saccharin). These sessions lasted 30 min and rats were returned to their home cages immediately afterwards.