2.6.Statistical analysis

IBM SPSS Statistics for Windows, Version 18.0 and GraphPad Prism software (San Diego, CA, USA) were used for statistical analysis and plotting. Differences of T lymphocyte subsets, SAMHD1 and HLA-DR levels among LSQD, WCSS and HD groups were analyzed by analysis of variance (ANOVA), and if there was a statistical difference among the three groups, differences between each two groups were compared using LDS method in pairs. Pearson or Spearman rank correlation was used to analyze the correlation between SAMHD1 expression and T cell activation. All statistical tests were two-sided and the hypothesis test level α was equal to 0.05.