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Isolation of immune cells from lung
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Lung tissues of mice were digested with collagenase and minced. The isolated cells were underlain with 3 ml lymphocyte separation solution (DAKEWE), centrifuged at 800 rcf for 30 min. The isolated cells were incubated for 2 h and adherent cells were harvested as lung macrophages. The cells suspended in the culture medium were harvested as lung lymphocytes.
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