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MAECs were transfected with specific siRNAs against ABCA1 or scrambled control siRNAs (Ctrlsi) according to the manufacturer’s instructions. After 6 h, cells were replenished with fresh endothelium medium plus growth factor supplement and cultured for an additional 24 h. These transfection procedures were repeated one time [29]. The knockdown efficiency induced by siRNA was confirmed by detection of ABCA1 proteins using WB analysis.
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